Internal control genes for quantitative RT-PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages
نویسندگان
چکیده
منابع مشابه
Expression pattern analysis of TomPRO2 and LaPA1 genes in tomato under in vitro salt stress by Semi-quantitative RT-PCR
The expression pattern of TomPRO2 and LaPA1 genes in two tomato (Lycopersicon esculentum) cultivars named as Isfahani and Shirazi under in vitro salt stress were investigated. Four to six weeks old in vitro grown seedlings were transferred on MS medium containing 0, 80 and 160 mM NaCl and untreated plants were used as control. RNA was extracted from root and leaf and then cDNA was synthesized. ...
متن کاملRelative quantitative RT-PCR protocol for TrkB expression in neuroblastoma using GAPD as an internal control.
An RT-PCR protocol for the relative quantitative measurement of TrkB transcripts using glyceraldehyde-3-phosphate-dehydrogenase (GAPD) transcripts as an internal control is described. Both TrkB and GAPD sequences were co-amplified in the exponential phase of amplification using 5'-biotinylated primers. The PCR products were subjected to PAGE, electro-transferred to nylon membrane and detected b...
متن کاملQuantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
The analysis of differences in gene expression is dependent on normalization using reference genes. However, the expression of many of these reference genes, as evaluated by quantitative RT-PCR, is upregulated in acute pancreatitis, so they cannot be used as the standard for gene expression in this condition. For this reason, we sought to identify a stable reference gene, or a suitable combinat...
متن کاملSemiquantitative RT-PCR analysis to assess the expression levels of iNOS in chicken macrophages
Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...
متن کاملSelection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
Background: Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls. For glioblastoma, the most common type of tumor in the central nervous system, there was no previous report regarding this issue. Results: Here we show that amongst seven freque...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2011
ISSN: 1756-0500
DOI: 10.1186/1756-0500-4-410